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Description

A spectrophotometer measures the number of photons emitted to estimate the intensity of light spectra absorbed and transmitted by a sample. This provides information on the amount of a compound in the sample.

Spectrophotometer

Quantity
  • Technical specifications Description
    Wavelength selection Monochromator
    Light source Xenon flash lamp
    Wavelength range 200–1,000 nm with 1 nm steps
    Readout range Up to 4 absorbance units
    Bandwidth <2.5 nm
    Linearity at 450 nm* 0–2.5 Abs, 2%
    Accuracy at 450 nm* 1.0% + 0.003 Abs (0–2.0 Abs), 2.0% (2.0–2.5 Abs)
    Precision at 450 nm* SD <0.003 Abs or CV <1.0%
    Plates 6- to 48-, 96-, and 6-384-well plates***, Thermo Scientific µDrop Plates
    Cuvette Dimensions (H x W x D): 40–58 x 12.5 x 12.5 mm

     

    Beam center height: 8.5 mm

    Beam window: ≥2 mm

    Measurement speed

     

    (starting from A1 back to A1)

    6 sec with 96-well plate

     

    10 sec with 384-well plate

    Plate shaking Linear
    Spectral scanning speed 10 sec from 200–1,000 nm with 1 nm steps
    Incubation range From ambient temperature +2C to 45˚C”.
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