A spectrophotometer measures the number of photons emitted to estimate the intensity of light spectra absorbed and transmitted by a sample. This provides information on the amount of a compound in the sample.
Spectrophotometer
Technical specifications
Description
Wavelength selection
Monochromator
Light source
Xenon flash lamp
Wavelength range
200–1,000 nm with 1 nm steps
Readout range
Up to 4 absorbance units
Bandwidth
<2.5 nm
Linearity at 450 nm*
0–2.5 Abs, 2%
Accuracy at 450 nm*
1.0% + 0.003 Abs (0–2.0 Abs), 2.0% (2.0–2.5 Abs)
Precision at 450 nm*
SD <0.003 Abs or CV <1.0%
Plates
6- to 48-, 96-, and 6-384-well plates***, Thermo Scientific µDrop Plates
